OCIMUM SANCTUM SEEDS BASED MATRIX FOR IMMOBILIZATION OF ALPHA-RHAMNOSIDASE

Authors
AFAQ Z YADAV KK YADAV KDS
Citation
Z. Afaq et al., OCIMUM SANCTUM SEEDS BASED MATRIX FOR IMMOBILIZATION OF ALPHA-RHAMNOSIDASE, Indian journal of chemistry. Sect. B: organic chemistry, including medical chemistry, 37(2), 1998, pp. 195-198
Citations number
13
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Indian journal of chemistry. Sect. B: organic chemistry, including medical chemistry → ACNP
ISSN journal
03764699
Volume
37
Issue
2
Year of publication
1998
Pages
195 - 198
Database
ISI
SICI code
0376-4699(1998)37:2<195:OSSBMF>2.0.ZU;2-#
Abstract
An Ocimum sanctum seed based matrix for immobilisation of enzymes has been developed. The alpha-rhamnosidase activity of naringinase has bee n immobilised on this newly developed matrix. K-m, pH and temperature optima of the immobilized enzyme using p-nitrophenyl-alpha-L-rhamnopyr anoside as the substrate have been determined and found to be 0.17 mM, 4.0 and 60 degrees C respectively.