OPTICAL RESOLUTION BY HIGH-PERFORMANCE CAPILLARY ELECTROPHORESIS - MICELLAR ELECTROKINETIC CHROMATOGRAPHY WITH SODIUM N-DODECANOYL-L-GLUTAMATE AND DIGITONIN

Optical resolution by micellar electrokinetic chromatography with sodi um N-dodecanoyl-L-glutamate (SDGlu) and with digitonin-sodium taurodeo xycholate (STDC) mixed micelles was investigated. Addition of sodium d odecyl sulphate, urea and methanol to SDGlu micellar solutions could g ive improved peak shapes and resolution. With SDGlu, phenylthiohydanto in (PTH) derivatives of five DL-amino acids (alpha-aminobutyric acid, methionine, norvaline, tryptophan and norleucine) were separated from each other and each pair of enantiomers was optically resolved. On the other hand, three PTH-DL-amino acids (norvaline, valine and alpha-ami nobutyric acid) were also successfully resolved with a digitonin-STDC- urea solution (pH 2.5).