PRODUCTION OF GALACTOOLIGOSACCHARIDES BY BETA-GALACTOSIDASE IMMOBILIZED ON GLUTARALDEHYDE-TREATED CHITOSAN BEADS

Authors
SHEU DC LI SY DUAN KJ CHEN CW
Citation
Dc. Sheu et al., PRODUCTION OF GALACTOOLIGOSACCHARIDES BY BETA-GALACTOSIDASE IMMOBILIZED ON GLUTARALDEHYDE-TREATED CHITOSAN BEADS, Biotechnology techniques, 12(4), 1998, pp. 273-276
Citations number
6
Categorie Soggetti
Biothechnology & Applied Migrobiology","Biochemical Research Methods
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
12
Issue
4
Year of publication
1998
Pages
273 - 276
Database
ISI
SICI code
0951-208X(1998)12:4<273:POGBBI>2.0.ZU;2-U
Abstract
beta-Galactosidase from Aspergillus oryzae, immobilized on glutaraldeh yde-treated chitosan beads, produced galactooligosaccharides (GOS) in a plug reactor. Maximum yields of GOS were 18, 21, 26 with lactose at 100, 200, 300 g/L, respectively. Yields of GOS in the immobilized enzy me system were always lower than that of free enzyme system. In a cont inuous reaction there was no significant loss of activity after 30 day s of operation.