POLYETHYLENE-GLYCOL IMPROVES THE PURIFICATION OF RECOMBINANT HUMAN TUMOR-NECROSIS-FACTOR DURING ION-EXCHANGE CHROMATOGRAPHY

Authors
FENG XL GU ZY JIN YT SU ZG
Citation
Xl. Feng et al., POLYETHYLENE-GLYCOL IMPROVES THE PURIFICATION OF RECOMBINANT HUMAN TUMOR-NECROSIS-FACTOR DURING ION-EXCHANGE CHROMATOGRAPHY, Biotechnology techniques, 12(4), 1998, pp. 289-293
Citations number
12
Categorie Soggetti
Biothechnology & Applied Migrobiology","Biochemical Research Methods
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
12
Issue
4
Year of publication
1998
Pages
289 - 293
Database
ISI
SICI code
0951-208X(1998)12:4<289:PITPOR>2.0.ZU;2-I
Abstract
Poly(ethylene glycol) (PEG) - either PEG 200, 600 or 4000 - when added to the buffer and feed solution gave nearly one-fold increase in the purification and about 100% recovery of recombinant human tumor necros is factor-alpha during anion-exchange chromatography. The optimum conc entration was 1% with the best result being achieved with PEG 200.