Af. Muttray et Ww. Mohn, RNA DNA RATIO AS AN INDICATOR OF METABOLIC-ACTIVITY IN RESIN ACID-DEGRADING BACTERIA/, Water science and technology, 37(4-5), 1998, pp. 89-93
Citations number
16
Categorie Soggetti
Water Resources","Environmental Sciences","Engineering, Civil
We investigated the relationship between the growth rate and the ratio
of RNA to DNA in of four resin acid degrading bacteria isolated from
a sequencing batch reactor (SBR). Chemical assays as well as slot blot
hybridizations with species-specific oligonucleotide probes were used
to quantify the nucleic acids. These slow-growing bacteria have a pos
itive linear correlation between growth rate and RNA/DNA ratio, simila
r to faster-growing bacteria like E. coli. We propose to use this corr
elation to measure metabolic activities of selected resin acid degradi
ng bacteria in the complex community of the SBR in situ using Species-
specific hybridization probes. preliminary experiments suggest that hy
bridization probes can be used to detect growth rate-dependent changes
in the RNA/DNA ratio. (C) 1998 IAWQ. Published by Elsevier Science Lt
d.