BIOAUGMENTATION OF SEQUENCING BATCH REACTORS FOR BIOLOGICAL PHOSPHORUS REMOVAL - COMPARATIVE RIBOSOMAL-RNA SEQUENCE-ANALYSIS AND HYBRIDIZATION WITH OLIGONUCLEOTIDE PROBES
Db. Oerther et al., BIOAUGMENTATION OF SEQUENCING BATCH REACTORS FOR BIOLOGICAL PHOSPHORUS REMOVAL - COMPARATIVE RIBOSOMAL-RNA SEQUENCE-ANALYSIS AND HYBRIDIZATION WITH OLIGONUCLEOTIDE PROBES, Water science and technology, 37(4-5), 1998, pp. 469-473
Citations number
11
Categorie Soggetti
Water Resources","Environmental Sciences","Engineering, Civil
Four laboratory-scale sequencing batch reactors (SBRs) were operated t
o evaluate whether bioaugmentation with Acinetobacter spp. can be used
to improve start-up and performance of enhanced biological phosphorus
removal (EBPR) systems. Two of the SBRs were bioaugmented during star
t-up by adding pure cultures of Acinetobacter spp., the third reactor
received an amendment of activated sludge from a laboratory-scale EBPR
system, and the fourth reactor, receiving no amendment, served as a c
ontrol. Various chemical parameters were measured to monitor the perfo
rmance of the four SBRS. Oligonucleotide probes of nested phylogenetic
specificity were designed to quantify the contribution of Acinetobact
er to EBPR. The probes were characterized for use in quantitative memb
rane hybridizations and fluorescent in situ hybridizations. Data from
hybridizations with samples collected from the SBRs show declining lev
els of Acinetobacter spp. over the experiment. All four reactors achie
ved significant phosphorus removal and 90% nitrification after three d
ays of operation. The results do not show a positive correlation betwe
en levels of Acinetobacter and successful EBPR. (C) 1998 IAWQ. Publish
ed by Elsevier Science Ltd.