DEVELOPMENT AND APPLICATION OF 16S RIBOSOMAL-RNA-TARGETED OLIGONUCLEOTIDE PROBE FOR DETECTION OF THE PHOSPHATE-ACCUMULATING BACTERIUM MICROLUNATUS-PHOSPHOVORUS IN AN ENHANCED BIOLOGICAL PHOSPHORUS REMOVAL PROCESS

Authors
KAWAHARASAKI M KANAGAWA T TANAKA H NAKAMURA K
Citation
M. Kawaharasaki et al., DEVELOPMENT AND APPLICATION OF 16S RIBOSOMAL-RNA-TARGETED OLIGONUCLEOTIDE PROBE FOR DETECTION OF THE PHOSPHATE-ACCUMULATING BACTERIUM MICROLUNATUS-PHOSPHOVORUS IN AN ENHANCED BIOLOGICAL PHOSPHORUS REMOVAL PROCESS, Water science and technology, 37(4-5), 1998, pp. 481-484
Citations number
9
Categorie Soggetti
Water Resources","Environmental Sciences","Engineering, Civil
Journal title
Water science and technology → ACNP
ISSN journal
02731223
Volume
37
Issue
4-5
Year of publication
1998
Pages
481 - 484
Database
ISI
SICI code
0273-1223(1998)37:4-5<481:DAAO1R>2.0.ZU;2-6
Abstract
A 16S rRNA-targeted oligonucleotide probe (MP2) specific for the phosp hate-accumulating bacterium (PAB) M. phosphovorus was designed and app lied to a sludge from an enhanced biological phosphorus removal (EBPR) process. Probes specific for defined phylogenetic groups and the poly phosphate staining dye, DAPI (4', 6-diamidino-2-phenylindol dihydrochl oride) were also used to analyze the activated sludge community. M. ph osphovorus was about 3% of the total bacteria in the EBPR sludge used. Proteobacteria belonging to the beta subclass were the most abundant. Many coccoid bacteria similar to M. phosphovorus were stained with DA PI. The percentage of PABs detected by DAPI stain was about 9% of the total bacteria. (C) 1998 IAWQ. Published by Elsevier Science Ltd.