Fl. Delosreyes et al., CHARACTERIZATION OF FILAMENTOUS FOAMING IN ACTIVATED-SLUDGE SYSTEMS USING OLIGONUCLEOTIDE HYBRIDIZATION PROBES AND ANTIBODY PROBES, Water science and technology, 37(4-5), 1998, pp. 485-493
Citations number
28
Categorie Soggetti
Water Resources","Environmental Sciences","Engineering, Civil
A quantitative method was developed for estimating Gordona mass in act
ivated sludge foam and mixed liquor samples. The technique involves in
situ hybridization with a genus-specific fluorescently labeled oligon
ucleotide probe calibrated on pure cultures of Gordona. The immunofluo
rescent technique of Hernandez et al. was modified to allow staining w
ith fluorescently labeled antibody and hybridization probes. The resul
ts of this technique were compared to those From membrane hybridizatio
n studies using radioactively-labeled oligonucleotide probes. Quantita
tive membrane hybridizations, in situ hybridizations, and antibody sta
ining resulted in significantly different levels of Gordona in activat
ed sludge foam, activated sludge mixed liquor, return activated sludge
, and anaerobic digester sludge. Simultaneous staining with labeled an
tibodies and oligonucleotide probes provide a definitive identificatio
n for Gordona, and represents a new approach for in situ studies of th
is organism's role in foaming. (C) 1998 IAWQ. Published by Elsevier Sc
ience Ltd.