OPTIMIZATION OF MEDIUM CONSTITUENTS AND FERMENTATION CONDITIONS FOR THE PRODUCTION OF L-GLUTAMIC ACID BY THE COIMMOBILIZED WHOLE CELLS OF MICROCOCCUS-GLUTAMICUS AND PSEUDOMONAS-REPTILIVORA

Statistical experimental design was used to optimize medium constituen ts and the conditions of fermentation, viz., temperature, pH and the t ime of fermentation. Higher yields of L-glutamic acid (37.1 kg/m(3)) w as obtained after optimizing medium components and the conditions of f ermentation. The optimal levels of medium components were: 61.5575 kg/ m(3) glucose, 7.3272 kg/m(3) urea and 1.783 mu g/dm(3) biotin. The opt imum productivity was achieved using optimized medium at the fermentat ion temperature of 33.7 degrees C, initial pH 7.74, and at the time of fermentation of 58.4 h.