HIGH-YIELD CHROMATOGRAPHIC PURIFICATION OF ENZYMATICALLY PRODUCED TYROSINE FRUCTOSYL ESTER

The synthesis of L-tyrosine fructosyl ester, from fructose and L-tyros ine methyl ester, was carried out by a transesterification reaction ca talyzed by alpha-chymotrypsin in water without any organic cosolvent. The yield was optimized by regulating the pH of the reaction medium an d a maximum yield of 63% was obtained. A two-step process of tyrosine fructosyl ester purification was proposed using adsorbent resin and ac tivated carbon. Different elutions and supports easily applicable to i ndustrial process have been studied. Solutions of tyrosine fructosyl e ster with a 96% purity and 70% recovery yield were obtained by chromat ography on a column of acrylic polyester resins with a solution of 0.5 M NaCl as eluant.