SUCCESSIVE CULTIVATION OF FUSARIUM-OXYSPORUM ON RICE CHAFF FOR ECONOMIC PRODUCTION OF FIBRINOLYTIC ENZYME

Production of fibrinolytic enzyme by successive solid state cultivatio n of Fusarium oxysporum on rice chaff (RC) was studied. From recycling 20 g dry RC six times by pure batch culture, an overall of 23200IU fi brinolytic enzyme was obtained. A total of 27400IU was obtained when t he same amount of RC was used for six cycles with repeated batch ferme ntation employing immobilized whole cells, which also resulted in 30% (w/w) loss by weight of the substrate, while only 8% (w/w) of RC was d egraded in the initial pure batch fermentation.