ACETYL-COA DECARBONYLASE SYNTHASE COMPLEX FROM ARCHAEOGLOBUS-FULGIDUS/

The acetyl-CoA decarbonylase/synthase (ACDS) multienzyme complex catal yzes the reversible cleavage and synthesis of acetyl-CoA in methanogen s. This report of the enzyme complex in Archaeoglobus fulgidus demonst rates the existence of a functional ACDS complex in an organism that i s not a methanogen. The A. fulgidus enzyme complex contained five subu nits of 89, 72, 50, 49.5, and 18.5 kDa, and it catalyzed the overall s ynthesis of acetyl-CoA according to the following reaction: CO2 + 2 Fd (red)(Fe2+) + 2 H+ + CH3 - H4SPt + CoA reversible arrow acetyl-CoA + H 4SPt + 2 Fd(ox)(Fe3+)+ H2O where Fd is ferredoxin, and CH3-H4SPt and H 4SPt denote N-5-methyl-tetrahydrosarcinapterin and tetrahydrosarcinapt erin, respectively.