KINETIC-PROPERTIES OF DNA MIGRATION UNDER CLAMPED HOMOGENEOUS ELECTRIC-FIELD CONDITIONS - DNA SIZE, MIGRATION VELOCITIES AND REORIENTATION TIME DETERMINED IN A SINGLE CLAMPED HOMOGENEOUS ELECTRIC-FIELD RUN

By analyzing DNA migrations per pulse at different pulse times we dete rmined the reorientation times and migration velocities, during and af ter DNA reorientation of molecules separated under different clamped h omogeneous electric field (CHEF) electrophoretic conditions. We obtain ed functions relating these parameters and DNA migration per pulse wit h running variables and DNA size. A statistical procedure that validat es the predictions of these functions was also proposed using identica l data for function fitness and validation. A method is proposed to es timate DNA size, reorientation time and migration velocities using the distance migrated by a DNA molecule in a single CHEF experiment. (C) 1998 Elsevier Science B.V.