COMPUTER-ASSISTED PH OPTIMIZATION FOR THE SEPARATION OF GEOMETRIC ISOMERS IN CAPILLARY ZONE ELECTROPHORESIS

With the rapid development of capillary electrophoresis, several worke rs have considered the theoretical pH optimization for the separation of geometric isomers. However, for samples composed of more than two i somers, these mathematical treatments lead only to an optimum pH range . In this work, the application of software based on the iterative com putation of the resolution as a function of pH was studied, in order t o have direct access to the optimum pH value for complex mixtures of i somers. The values deduced were compared with the experimental values for acids and bases.