FREE-RADICAL GENERATORS CAUSE CHANGES IN ENDOTHELIAL AND INDUCIBLE NITRIC-OXIDE SYNTHASES AND ENDOTHELIN-1 IMMUNOREACTIVITY IN ENDOTHELIAL-CELLS FROM HYPERLIPIDEMIC RABBITS
G. Aliev et al., FREE-RADICAL GENERATORS CAUSE CHANGES IN ENDOTHELIAL AND INDUCIBLE NITRIC-OXIDE SYNTHASES AND ENDOTHELIN-1 IMMUNOREACTIVITY IN ENDOTHELIAL-CELLS FROM HYPERLIPIDEMIC RABBITS, MOLECULAR GENETICS AND METABOLISM, 63(3), 1998, pp. 191-197
Citations number
37
Categorie Soggetti
Genetics & Heredity","Medicine, Research & Experimental",Biology
Reactive oxygen species (ROS) play an important role in the damage of
vascular endothelium during atherogenesis and impaired endothelium-dep
endent vasorelaxation, We have studied the effect of two ROS generator
s (H2O2 and menadione) and one of the most potent antioxidants (morin)
on the double immunofluorescent staining of endothelial cells (EC) fr
om both Watanabe Heritable Hyperlipidemic (WHHL) and New Zealand White
(NZW) rabbits in primary cultures using antibodies against endothelin
-1 (ET-1), endothelial (eNOS), and inducible nitric oxide synthase (iN
OS). In aortic EC from normal rabbits, ROS decreased the immunoreactiv
ity of eNOS and ET-1 and this effect was significantly reversed by mor
in. In atherosclerotic rabbits, ROS had the same effect on the immunor
eactivity of eNOS and ET-1 but also induced the expression of iNOS imm
unoreactivity. In general, the cells from WHHL rabbits were less sensi
tive to the protective effects of morin and more sensitive to the effe
cts of ROS. It thus appears that the protective effect of morin may be
due to neutralization of ROS and may be considered for the treatment
of early stages of atherosclerosis, before macroscopic lesions have oc
curred. (C) 1998 Academic Press.