CHARACTERIZATION OF VP6 SUBGROUP, VP7 AND VP4 GENOTYPE OF ROTAVIRUS STRAINS IN LUSAKA, ZAMBIA

In a previous study, rotavirus infection was determined in young child ren at the University Teaching Hospital in Lusaka. In this study, sele cted rotavirus strains were characterized by monoclonal antibody assay to the VP6 subgroup antigen present and by hybridization analysis of the VP7 and VP4 genes carried by the virus. The majority of the strain s were characterized as a VP6 subgroup II, VP7 serotype G1 strain with a long electropherotype and bearing the VP4 P8 genotype. A further fo ur minor rotavirus strains with a long RNA electropherotype and subgro up II antigen were also observed to be circulating bearing G1 or G4 VP 7 genes and the VP4 P8 genotype. Two electrophoretic strains with diff ering short RNA electropherotypes and subgroup I antigenicity were als o present. These strains hybridized to the VP7 type G2 and VP4 P4 geno type probes.