HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF BISOPROLOL AND POTENTIAL IMPURITIES

An HPLC method capable of determining bisoprolol in bulk substance in the presence of its synthetic intermediates, which are potential impur ities, is described. To choose the optimum conditions the behaviour of the compounds on octadecylsilica columns with acetonitrile-phosphate buffer mobile phases was studied. The influence of the acetonitrile an d buffer concentrations and the pH of the mobile phase on retention wa s investigated. The results indicate that hydrophobic and silanophilic interactions contribute to the retention of the compounds.