RAPID CAPILLARY GEL-ELECTROPHORESIS OF PROTEINS

The rapid separation of sodium dodecyl sulphate-protein complexes acco rding to their molecular masses (M(r)) by capillary gel electrophoresi s is described. Using commercial equipment, standard proteins with M(r ) in the range 29000-97400 were resolved to the baseline in less than 2 min by utilizing a separation distance of 7 cm. A linear relationshi p between migration time and log M(r) was found and rapid determinatio n of the molecular mass of light and heavy chains of human immunoglobu lin G is reported. The results are compared with applications using lo nger separation distances, showing that rapid and efficient analysis a nd adequate resolution can be obtained by using short separation dista nces.