ESTABLISHMENT AND CHARACTERIZATION OF BONE-MARROW STROMA-DEPENDENT LEUKEMIA-CELL LINE, HB-1

Radiation-induced M361 leukemia bearing mice (M361 mice) show characte ristics of acute myelogenous leukemia (AML) with granulocytosis. Granu locyte-macrophage colony forming unit (CFU-GM) increased in the bone m arrow and spleen and high activity of colony stimulating factor (CSF) was found in the sera of M361 mice. A cell line (HB-1) was established from the spleen cells of M361 mice. Injection of HB-1 cells induced a similar leukemic response as M361 in syngeneic mice. HB-1 cells did n ot survive in the suspension culture, but proliferated when cultured o n hemopoietic supportive stromal cells (MS-10). HB-1 cells appear to b e strictly dependent on the hemopoietic supportive bone marrow stroma, which would provide a useful model for the study of cell-cell interac tions between hemopoietic cells and marrow stromal cells. (C) 1998 Els evier Science Ireland Ltd. All rights reserved.