BIOENERGETICS OF CONTRACTING SKELETAL-MUSCLE AFTER PARTIAL REDUCTION OF BLOOD-FLOW

The purpose of this study was to examine the bioenergetics and regulat ion of O-2 uptake ((V) over dot O-2) and force production in contracti ng muscle when blood flow was moderately reduced during a steady-state contractile period. Canine gastrocnemius muscle (n = 5) was isolated, and 3-min stimulation periods of isometric, tetanic contractions were elicited sequentially at rates of 0.25, 0.33, and 0.5 contractions is (Hz) immediately followed by a reduction of blood flow [ischemic (I) condition] to 46 +/- 3% of the value obtained at 0.5 Hz with normal bl ood flow. The (V) over dot O-2 of the contracting muscle was significa ntly (P < 0.05) reduced during the I condition [6.5 +/- 0.8 (SE) ml.10 0 g(-1).min(-1)] compared with the same stimulation frequency with nor mal flow (11.2 +/- 1.5 ml.100 g(-1).min(-1)), as was the tension-time index (79 +/- 12 vs. 123 +/- 22 N.g(-1).min(-1), respectively). The ra tio of (V) over dot O-2 to tension-time index remained constant throug hout all contraction periods. Muscle phosphocreatine concentration, AT P concentration, and lactate efflux were not significantly different d uring the I condition compared with the 0.5-Hz condition with normal b lood flow. However, at comparable rates of (V) over dot O-2 and tensio n-time index, muscle phosphocreatine concentration and ATP concentrati on were significantly less during the I condition compared with normal -flow conditions. These results demonstrate that, in this highly oxida tive muscle, the normal balance of O-2 supply to force output was main tained during moderate ischemia by downregulation of force production. In addition, 1) the minimal disruption in intracellular homeostasis a fter the initiation of ischemia was likely a result of steady-state me tabolic conditions having already been activated, and 2) the differenc e in intracellular conditions at comparable rates of (V) over dot O-2 and tension-time index between the normal flow and I condition may hav e been due to altered intracellular O-2 tension. .