PURIFICATION OF PASII PMP22 - AN EXTREMELY HYDROPHOBIC GLYCOPROTEIN OF PNS MYELIN MEMBRANE/

PASII/PMP22 protein (mol. wt 22 kDa, pI 8.8) is an abundant and extrem ely hydrophobic glycoprotein of PNS myelin which is solubilized effect ively by SDS. In humans, this protein is involved in hereditary neurop athies. Here we describe a simple method for purification of PASII/PMP 22, suitable for crystallization trials. We usually obtained 10-20 mg PASII/PMP22 from 10 g bovine spinal roots. It is notable, that the ori ginal protocol was designated for purification of PO myelin glycoprote in, and purification of PASII/PMP22 is a bonus. Extensive crystallizat ion trials are in progress. (C) 1998 Rapid Science Ltd.