GRADIENT HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF ENKEPHALIN PEPTIDES, THEIR METABOLITES, AND ENZYME-INHIBITORS USING COMBINED ULTRAVIOLET AND ELECTROCHEMICAL DETECTION - II - APPLICATION TO OCULAR PERMEABILITY STUDIES IN-VITRO
Vp. Ranta et al., GRADIENT HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF ENKEPHALIN PEPTIDES, THEIR METABOLITES, AND ENZYME-INHIBITORS USING COMBINED ULTRAVIOLET AND ELECTROCHEMICAL DETECTION - II - APPLICATION TO OCULAR PERMEABILITY STUDIES IN-VITRO, Journal of chromatography B. Biomedical sciences and applications, 709(1), 1998, pp. 1-10
Citations number
12
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical sciences and applications
A gradient HPLC method with combined ultraviolet (UV) and electrochemi
cal detection (ED) was used to study the ocular permeability of [D-Ala
(2)]-methionine enkephalinamide (MEA) in vitro. Coulometric ED was sel
ective for MEA and its tyrosine-containing metabolites with quantitati
on Limits between 20 and 60 nM (1.0-3.0 pmol per 50-mu l injection), w
hereas UV detection at 205 nm allowed the determination of several aro
matic metabolites and enzyme inhibitors with quantitation limits betwe
en 40 and 500 nW (2.0-25.0 pmol), This method was capable of detecting
permeability of MEA and metabolite formation in the cornea and conjun
ctiva in vitro. Furthermore, effects of aminopeptidase inhibitor besta
tin and enkephalinase inhibitor SCH 39370 on permeation and metabolism
of MEA could be determined. (C) 1998 Elsevier Science B.V.