STEREOSELECTIVE DETERMINATION OF R,S-2-[4-(3-METHYL-2-THIENYL)PHENYL]PROPIONIC ACID AND ITS TAURINE CONJUGATES IN DOG URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

Citation
T. Konishi et al., STEREOSELECTIVE DETERMINATION OF R,S-2-[4-(3-METHYL-2-THIENYL)PHENYL]PROPIONIC ACID AND ITS TAURINE CONJUGATES IN DOG URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Journal of chromatography B. Biomedical sciences and applications, 709(1), 1998, pp. 105-111
Citations number
14
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical sciences and applications
ISSN journal
13872273 → ACNP
Volume
709
Issue
1
Year of publication
1998
Pages
105 - 111
Database
ISI
SICI code
0378-4347(1998)709:1<105:SDOR>2.0.ZU;2-E
Abstract
Two high-performance liquid chromatographic methods for the stereosele ctive determination of R,S-2-[4-(3-methyl-2-thienyl)-phenyl]propionic acid (R,S-MTPPA), a new anti-inflammatory agent, and its taurine conju gates (R,S-MTPPA-TAU) in dog urine have been developed and validated. The urine samples were subjected to solid extraction or TLC preparatio n, then R,S-MTPPA and R,S-MTPPA-TAU were separated on Chiralcel OD and Chiral AGP columns, respectively, with ultraviolet absorbance detecti on at 272 nm. The dose-response relationships for enantiomers of R,S-M TPPA and R,S-MTPPA-TAU were linear in the concentration ranges of 0.5- 50 (r>0.9993) and 5-200 mu g/ml (r>0.9982), respectively. Recoveries o f all tested enantiomers from dog urine were roughly 90% within the ab ove concentration ranges. Intra-and inter-day reproducibilities were s ufficient for metabolic studies. These methods were applied to stereos elective determination of the enantiomers in dog urine after administr ation of either S- or R-MTPPA. (C) 1998 Elsevier Science B.V.