In this study we investigated the level of 8-oxo-2 '-deoxyguanosine (8-oxod
G) in DNA of Cardamine pratensis plants subjected to different growth condi
tions trying to answer the question whether factors like light and water ac
cessibility or low temperature may have an impact on the total DNA oxidativ
e damage. The level of this modified nucleoside was determined using HPLC c
oupled to UV absorbance and electrochemical detection (HPLC-UV-EC). We did
not observe any statistically significant differences in 8-oxodG level betw
een DNA of etiolated and light exposed plants as well as between DNA of reg
ularly watered and drought-subjected plants. In contrast, we have shown tha
t chilling (1 degrees C for 28 h) brings about the increase of 8-oxodG leve
l in DNA.