P. Riso et al., Does tomato consumption effectively increase the resistance of lymphocyte DNA to oxidative damage?, AM J CLIN N, 69(4), 1999, pp. 712-718
Background: Lycopene, the main carotenoid in tomato, has been shown to be a
potent antioxidant in vitro. However, there is no significant evidence of
its antioxidant action in vivo.
Objective: We evaluated the effect of tomato intake on plasma carotenoid co
ncentrations and lymphocyte resistance to oxidative stress.
Design: Ten healthy women (divided into 2 groups of 5 subjects each) ate a
diet containing tomato puree (providing 16.5 mg lycopene) and a tomato-free
diet for 21 d each in a crossover design. Before and after each diet perio
d, plasma carotenoid concentrations and primary lymphocyte resistance to ox
idative stress (evaluated by means of single-cell gel electrophoresis) were
analyzed.
Results: After the first 21-d experimental period, total plasma lycopene co
ncentrations increased by 0.5 mu mol/L (95% CI: 0.14, 0.87) in the group th
at consumed the tomato diet and decreased by 0.2 mu mol/L. (95% CI: -0.11,
-0.30) in the group that consumed the tomato-free diet (P < 0.001). Tomato
consumption also had an effect on cellular antioxidant capacity: lymphocyte
DNA damage after ex vivo treatment with hydrogen peroxide decreased by 33%
(95% CI: 0.8%, 61%; P < 0.05) and by 42% (95% CI: 5.1%, 78%; P < 0.05)in t
he 2 groups of subjects after consumption of the tomato diet.
Conclusion: The consumption of tomato products may reduce the susceptibilit
y of lymphocyte DNA to oxidative damage.