Electrochemical behavior of myoglobin at a thiamazole modified silver electrode

Thiamazole is covalently attached onto the surface of a silver electrode to form a chemically modified one, which is long-lived and stable. It shows t he capability of selectively complexing myoglobin and can be applied as a s elective biosensor for this compound. Myoglobin is accumulated onto the CME at 0.32V (vs.SCE). After exchanging the medium, it is determined by differ ential pulse stripping voltammetry. A cathodic stripping peak is obtained b y scanning the potential from 0.32V to 0.0V. The peak currents are linearly proportional to the concentrations of the protein in the range of 0.5 simi lar to 5.0mg/ml. The relative standard deviation is 6.4 % for 6 successive determinations of 3.0mg/ml myoglobin.