SEPARATION OF ACIDIC PROTEINS BY CAPILLARY ZONE ELECTROPHORESIS AND SIZE-EXCLUSION HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY - A COMPARISON

Successful separations of alcohol dehydrogenase (pI=5.4), beta-amylase (pI=5.2) and albumin (pI=4.7) by capillary electrophoresis in uncoate d fused-silica capillaries are reported. Different electrophoretic con ditions, consisting in variation of temperature, applied voltage and i onic strength of the buffer used as electrolyte, were tested in order to compare the separation efficiency, resolution and selectivity of th e acidic proteins. The results were compared with those obtained by si ze-exclusion chromatography. Rinsing of the capillary between runs, in order to eliminate adsorbed proteins, can shorten its useful lifetime .