HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF PLASMA TRIGLYCERIDE TYPE COMPOSITION IN A NORMAL POPULATION OF BARCELONA - RELATIONSHIP WITH AGE, SEX AND OTHER PLASMA-LIPID PARAMETERS

A coupled TLC-HPLC procedure is proposed for the separation and determ ination of plasma triglycerides. The method was tested by application to plasma samples corresponding to a normal population of Barcelona (S pain). Eighteen different triglyceride types were identified and their relative proportions were established, in order to give a ''normal pr ofile'' for men and women. Sex-related differences (p < 0.05) were onl y found for dioleostearin and palmitodilinolein + linoleooleopalmitole in (LLP + LOPa). A correlation study showed that palmitodiolein and to tal cholesterol levels increase with age, whereas LLP-LOPa decreases i n men and palmitolinoleoolein + palmitooleopalmitolein in women.