Ja. Alarcon et Mc. Alvarez, Genetic identification of sparid species by isozyme markers: application to interspecific hybrids, AQUACULTURE, 173(1-4), 1999, pp. 95-103
The successful performance of Sparus aurata in Mediterranean aquaculture ha
s prompted the domestication of other sparid species and the production of
their interspecific hybrids. Allozyme markers have proved to be very powerf
ul to study genetic variation at interspecific level and have been applied
to differentiate the sparid species: Sparus aurata, Pagrus pagrus, Pagellus
bogaraveo, Diplodus sargus, Diplodus puntazzo, Lithognatus mormyrus, Dente
x dentex and Spondyliosoma cantharus. Seventeen enzymes, revealing 24 loci
were suitable for comparison purposes. Among them, only the AK * locus does
not allow differentiation of species, while the remaining revealed qualita
tive allelic differences in a variable number of species. The most resoluti
ve loci were: ENDOP *, EST *, G3PDH *, and LDH-1 *, which discriminated 24
out of 28 species pairs in a dual species comparison matrix. A practical ap
plication of this study, was to demonstrate the hybrid nature of the offspr
ing resulting from crossing female S. aurata and male P. pagrus. The ADH *
locus, revealed from liver, proved to be very resolutive in terms of band s
eparation. In addition the EST * showed to be very convenient, as it can be
revealed from fin and mucus, avoiding sacrificing the animals. (C) 1999 El
sevier Science B.V. All rights reserved.