DETERMINATION OF SODIUM 5,6-BENZYLIDENE-L-ASCORBATE AND RELATED-COMPOUNDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

A simple high-performance liquid chromatographic process with ultravio let detection was established to determine ascorbic acid, benzoic acid , benzaldehyde and the two different diastereomers of sodium 5,6-benzy lidene-L-ascorbate. The five substances were reproducibly separated on a reversed-phase C-18 column with gradient elution with an acetate bu ffer (pH 6.85)-methanol system. This method was applied to the determi nation of the concentrations of these substances in various biological fluids after their extraction and deproteinization with acetonitrile.