SEPARATION OF ANILINE DERIVATIVES BY MICELLAR ELECTROKINETIC CHROMATOGRAPHY

The separation of ten aniline derivatives was investigated by micellar electrokinetic chromatography. The pH dependence of their migration b ehaviour was determined. The capacity factor for each aniline derivati ve was calculated and its pH dependence was elucidated from the pK(a) value of the aniline derivative. In the case of p-anisidine, the pH de pendence can be explained by a simple model. The complete separation o f ten aniline derivatives was achieved by optimizing the pH and the co ncentration of sodium dodecyl sulphate, and by adding gamma-cyclodextr in. Separation of diphenylamine and N-nitrosodiphenylamine, which cann ot be easily distinguished by gas chromatography because of thermal de gradation, was achieved.