ANALYSIS OF N-ACYL AMINONAPHTHALENE SULFONIC-ACID DERIVATIVES WITH POTENTIAL ANTI-HUMAN-IMMUNODEFICIENCY-VIRUS ACTIVITY BY THIN-LAYER CHROMATOGRAPHY AND FLAME IONIZATION DETECTION

A method for checking the purity of N-acyl aminonaphthalene disulphoni c acid derivatives was required for a systematic study of the anti-hum an immunodeficiency virus activity of these agents. We describe the us e of thin-layer chromatography and flame ionization detection for the separation of these compounds, which are difficult to analyse by conve ntional methods. All the samples were prepared in methanol solutions ( 1 mu l) containing 5 mu g of aminonaphthalene derivative. These sample s were applied to each type SIII Chromarod by a single injection and d eveloped with pure methanol or a methanol-chloroform-ammonium hydroxid e (35:55:10, v/v/v) solvent system.