Interaction of S-SCAM with neural plakophilin-related armadillo-repeat protein/delta-catenin

Synaptic scaffolding molecule (S-SCAM) is a multiple PDZ domain-containing protein, which interacts with neuroligin, a cell adhesion molecule, and the NMDA receptor. In this study, we searched for S-SCAM-interacting proteins and obtained a neural plakophilin-related armadillo-repeat protein (NPRAP)/ delta-catenin, NPRAP/delta-catenin bound to the last PDZ domain of S-SCAM v ia its carboxyl-terminus in three different cell-free assay systems, was co immunoprecipitated with S-SCAM from rat crude synaptosomes, and was localiz ed at the excitatory synapses in rat hippocampal neurons. NPRAP/delta-caten in may be implicated in the molecular organization of synaptic junctions th rough the interaction with S-SCAM, (C) 1999 Academic Press.