The IaIA invasion gene of Bartonella bacilliformis encodes a (di)nucleoside polyphosphate hydrolase of the MutT motif family and has homologs in other invasive bacteria

The product of the ialA invasion gene of Bartonella bacilliformis has been expressed as a thioredoxin fusion protein, it is a (di)nucleoside polyphosp hate hydrolase of the MutT motif protein family with strong sequence simila rity to plant diadenosine tetraphosphate hydrolases. It hydrolyses nucleosi de and dinucleoside polyphosphates with four or more phosphate groups, alwa ys producing an NTP as one product. Diadenosine tetraphosphate (Ap(4)A) is the preferred substrate with a K-m of 10 mu M and a k(cat) of 3.0 s(-1). It is inhibited by Ca2+ and F- (K-i = 80 mu M). Hydrolysis of Ap(4)A in (H2O) -O-18 yielded [O-18]AMP as the only labelled product. in terms of sequence, reaction mechanism and properties, IalA is very similar to eukaryotic Ap(4 )A hydrolases, and unlike previously described bacterial Ap(4)A hydrolases. : Homologs are present in the genomes of other invasive pathogens. They may function: to reduce stress-induced dinucleotide levels during invasion and so enhance pathogen survival. (C) 1999 Academic Press.