Al. Serafino et al., Cellular uptake and delivery monitoring of liposome/DNA complexes during in vitro transfection of CFTR gene, BIOC MOL B, 47(2), 1999, pp. 337-344
The cellular uptake and distribution of cationic Liposomes Dc-Chol/DOPE-CFT
R gene complexes were assessed by electronic and confocal laser scanner mic
roscopy (CLSM) for the CFTR gene transfer to human adenocarcinoma and trach
eal epithelial cell lines. Cationic lipid forms unilamellar and multilamell
ar vesicles capable of rapid and efficient transport of gene into target ce
lls. The number of fluorescent complexes was increasing with time in cells
up to 6 hours showing a punctate and homogeneous DNA distribution in the cy
toplasmatic and nuclear compartments, including the nucleolus. No significa
nt difference in the biochemical and cellular behavior was observed between
the investigated system and other systems previously tested. This study ad
ds new insights into the CFTR cationic liposome-mediated gene delivery.