Synthesis of an antisense oligonucleotide targeted against C-raf kinase: Efficient oligonucleotide synthesis without chlorinated solvents

Authors
Krotz, AH Cole, DL Ravikumar, VT
Citation
Ah. Krotz et al., Synthesis of an antisense oligonucleotide targeted against C-raf kinase: Efficient oligonucleotide synthesis without chlorinated solvents, BIO MED CH, 7(3), 1999, pp. 435-439
Citations number
24
Categorie Soggetti
Chemistry & Analysis
Journal title
BIOORGANIC & MEDICINAL CHEMISTRY
ISSN journal
09680896 → ACNP
Volume
7
Issue
3
Year of publication
1999
Pages
435 - 439
Database
ISI
SICI code
0968-0896(199903)7:3<435:SOAAOT>2.0.ZU;2-L
Abstract
It is demonstrated that a solution of dichloroacetic acid in toluene remove s dimethoxytrityl groups from the 5'-terminus of an antisense phosphorothio ate oligodeoxyribonucleotide (ISIS 5132/CGP69846A) during synthesis on soli d support cleanly and efficiently. It is therefore suggested to replace hea lth hazardous dichloromethane which is typically used in oligonucleotide sy nthesis as solvent for DMTr-removal by toluene. (C) 1999 Elsevier Science L td. All rights reserved.