A new solid-phase synthesis of oligonucleotides 3 '-conjugated with peptides

Authors
De Napoli, L Messere, A Montesarchio, D Piccialli, G Benedetti, E Bucci, E Rossi, F
Citation
L. De Napoli et al., A new solid-phase synthesis of oligonucleotides 3 '-conjugated with peptides, BIO MED CH, 7(2), 1999, pp. 395-400
Citations number
17
Categorie Soggetti
Chemistry & Analysis
Journal title
BIOORGANIC & MEDICINAL CHEMISTRY
ISSN journal
09680896 → ACNP
Volume
7
Issue
2
Year of publication
1999
Pages
395 - 400
Database
ISI
SICI code
0968-0896(199902)7:2<395:ANSSOO>2.0.ZU;2-7
Abstract
A convenient 'on line' solid-phase synthesis of oligonucleotides conjugated at the 3'-end with peptides by means of a polymeric support linking the fi rst nucleoside via the base has been developed. A 17-mer designed for antis ense experiments against HIV-1, linking at the 3'-terminus the tripeptide G ly-Gly-His, was prepared in good yields and characterized by MALDI-TOF mass spectrometry. (C) 1999 Elsevier Science Ltd. All rights reserved.