Km. Maloney et al., Binding and two-dimensional crystallization of streptavidin at the air/water interface via engineered Cu-IDA chelator lipids, COORD CH RE, 183, 1999, pp. 3-18
Metal-chelating iminodiacetate (IDA) lipid monolayers at the air/water inte
rface have been engineered to promote the 2D crystallization of proteins wh
ich display solvent-accessible histidines. The physical properties of IDA l
ipid monolayers have been specifically tailored to allow the binding and 2D
assembly of added proteins. Fluorescently labeled IDA lipid monolayers all
ow us to monitor the formation of the protein crystals optically. Here we r
eview our efforts in the interfacial crystallization of streptavidin as mon
itored using fluorescence, Brewster angle and transmission electron microsc
opy and discuss future challenges in this area. (c) 1999 Elsevier Science S
.A. All rights reserved.