Effect of cyclosporine A on cytochrome P-450-mediated drug metabolism in the partially hepatectomized rat

Despite its hepatotoxic potential, cyclosporine A (CsA) has been reported t o positively influence compensatory liver growth. To probe the physiologica l consequences of CsA on the recovery of liver function, studies were initi ated in the 2/3 partially hepatectomized (PHx) rat, taking the recovery of cytochromes P-450-dependent drug metabolism as primary outcome. CsA was adm inistered at a dose of 3.33 mg/kg/day for 10 days. Drug metabolism was eval uated by the recovery of (CO2)-C-14 after administration of isotopically la beled model drugs and by studying the expression of the P-450 transcripts i nvolved in their biotransformation before and 24 to 96 h after PHx. Before PHx, neither the steady-state mRNA nor the in vivo disposition of caffeine (CYP1A2), erythromycin (CYP3A2 and 3A1), or aminopyrine (CYP2B1 and 2C11) w ere influenced by CsA, Studies 24 h after PHx revealed a 29 to 39% reductio n in the elimination of [C-14]aminopyrine and [C-14]erythromycin, which was unaffected by CsA. Their metabolism at 48 to 96 h after PHx also remained unaffected by CsA. By contrast, postPHx, [C-14]caffeine elimination decreas ed to a level closely proportional to the loss in liver mass. In addition, CsA accelerated the recovery and/or prevented the decrease of caffeine elim ination 24 h after PHx but not at later time points, indicating an early, b ut unsustained, beneficial effect of CsA on the recovery of CYP1A2-mediated activities. These data show that at the critical time of greatest loss in liver mass, CsA has only a selective influence on the biotransformation of cytochrome P-450 protein-dependent activities and that its effect on the re generation process does not translate into an overall accelerated recovery of the hepatic drug-metabolizing function.