The effect of ATP-MgCl2 on lipid peroxidation in ischemic and reperfused rabbit kidney

Oxygen metabolites formed during reperfusion of ischemic kidneys prevent re covery of renal function after short periods of renal ischemia. The adminis tration of ATP-MgCl2 is beneficial to the survival of animals after hemorrh agic shock, severe burns, septicemia-peritonitis, post-ischemic hepatic fai lure, bowel ischemia, and endotoxic shock. In this study, the effect of ATP -MgCl2 on lipid peroxidation and its curative effect were evaluated by meas uring the decomposition products of lipid peroxidation, detected as thiobar bituric-acid reactive substances in homogenized kidney tissues in ischemic and reperfused rabbit kidneys. Ischemia was performed by clamping the right renal artery for 60 minutes followed by 30 minutes of reperfusion. Thirty- six rabbits were classified into 6 groups containing 6 rabbits in each. In the first group, no renal ischemia-reperfusion (I-R) was designed (Sham gro up), the right kidney was removed 90 minutes later. In the second group, I- R was established but nothing given. Saline 0.25 cc/kg was given into the r ight renal artery in group 3 two minutes before ischemia, and in group 4 tw o minutes before reperfusion. ATP-MgCl2 17.5 mu mol/kg (0.25 cc/kg) was giv en two minutes before ischemia in group 5, and before reperfusion in group 6. The right kidneys of the rabbits were removed and thiobarbituric-acid re active substances in the homogenates were measured. In addition, histopatho logical evaluation was performed. High lipid peroxidation products were rec orded in groups 2-5, whereas in group 6, these levels were low similar to t hose obtained in Sham group (76.72 +/- 1.01 nmol/g tissue). On histopatholo gical evaluation, a considerable cell damage resulting from I-R trauma espe cially in proximal; tubules was observed. In groups which were under saline effect, no histopathological damage was found. Histophatological preservat ion was better in group 6 rather than in group 5. The results of this study indicate that ATP-MgCl2 is remarkably effective for preventing the lipid p eroxidation if given before reperfusion but not before ischemia in experime ntal I-R injury in rabbit kidneys.