Mm. Deoliveira et al., DETECTION OF ANTIVIRAL AND ANTITUMORAL FRACTIONS OF CHENOPODIUM AMARANTICOLOR LEAF EXTRACT, Microbios, 76(309), 1993, pp. 213-221
Based on the fact that Chenopodium amaranticolor extracts showed inhib
itory activity against tobacco mosaic virus (TMV) and Ehrlich tumour (
EA), tests were carried out to investigate whether the antiviral and a
ntitumoral activity were caused by the same compounds. When the extrac
t was purified by CM Sephadex C-25 column, after precipitation with 90
% ammonium sulphate, twenty active fractions against TMV and two pools
of fractions active against EA were obtained. Only one fraction with
high absorbance values at 260 and 280 nm was able to inhibit both TMV
and EA. When the extract was purified by Bio Gel P-60 column two activ
e fractions against TMV and EA were obtained, suggesting that they wer
e contained in the 0.01 M fraction of the CM Sephadex column. It is su
ggested that C. amaranticolor leaf extract contained at least two prot
ein-like substances manifesting antiviral and antitumoral activity.