Effects of polysulfonate derivative (GL 522-Y-1) on coagulation in vitro and thrombosis in vivo

We have investigated the influence of a polysulfonate derivative - GL 522-Y -1 - on platelet-induced thrombin generation time, platelet adhesion to sil iconized glass, platelet aggregation induced by collagen and ADP, on aPTT, PT and TT in vitro and studied its antithrombotic effect in an animal model of thrombosis in vivo. In vitro, GL 522-Y-1 caused inhibition of ADP- and collagen-induced aggregation. In a dose-dependent manner this compound inhi bited PITT, aPTT, and PT. GL-522-Y-1 did not prolong thrombin time. GL 522- Y-1 inhibited in vivo the laser-induced thrombus formation after intravenou s and oral administration. On the basis of its unique antithrombotic proper ties, GL 522-Y-1 seems to open a new pathway in the field of antithrombotic s.