Effects of lipopolysaccharide on endothelial cell adhesion molecule expression in interleukin-10 deficient mice

Interleukin (IL)-10 is known to inhibit the production of proinflammatory c ytokines by macrophages suggesting that endogenous IL-10 may act as an anti ; inflammatory agent. Because endothelial cell adhesion molecules (ECAMs) p lay a key role in the recruitment of leukocytes into tissue in response to an inflammatory stimulus (i.e., lipopolysaccharide (LPS)) and the following cytokine production, we wished to assess the importance of IL-IO as an end ogenous modulator of ECAM expression using IL-10 deficient mice. Constituti ve and LPS-stimulated expression of intercellular adhesion molecule (ICAM)- 1, vascular cell adhesion molecule (VCAM)-1 and E-selectin were measured in wild type C57BL/6 and IL-10 deficient mice with no signs of active enteroc olitis, using the dual radiolabeled monoclonal antibody technique. We found that constitutive expression of these ECAMs did not differ between IL-10 d eficient and WT mice for all organs tested. However, we demonstrated larger increments in LPS-induced expression of ICAM-1 and VCAM-1 in the vasculatu re of the small intestine in IL-IO deficient mice compared to WT mice. Thes e findings suggest that endogenous IL-10 does not modulate constitutive or LPS-induced expression of ECAMs in most tissues, however it does appear to play an inhibitory role in LPS-stimulated expression of ICAM-1 and VCAM-1 i n the intestinal vasculature.