Inducible nitric oxide synthase activity by interferon-gamma-producing cells in human radicular cysts

Citation
O. Takeichi et al., Inducible nitric oxide synthase activity by interferon-gamma-producing cells in human radicular cysts, INT ENDOD J, 32(2), 1999, pp. 124-130
Citations number
21
Categorie Soggetti
Dentistry/Oral Surgery & Medicine
Journal title
INTERNATIONAL ENDODONTIC JOURNAL
ISSN journal
01432885 → ACNP
Volume
32
Issue
2
Year of publication
1999
Pages
124 - 130
Database
ISI
SICI code
0143-2885(199903)32:2<124:INOSAB>2.0.ZU;2-4
Abstract
Aim In this study, the interaction of interferon-gamma-(IFN-gamma) and indu cible nitric oxide synthase (INOS)-producing cells in human radicular cysts were investigated. Methodology Inflamed periapical tissues were obtained from patients at the time of endodontic surgical treatments and were cut into two pieces. After fixing with acetone or 4% paraformaldehyde in phosphate-buffered saline. 5- m-thick paraffin and cryostat sections were prepared. The paraffin sections of the inflamed tissues were evaluated histologically with haematoxylineos in stains, The specimens diagnosed as radicular cysts were then examined by immunostaining. Immunohistochemistry for iNOS and fluoresence microscopy f or IFN-gamma using the cryostat sections were performed with a mixture of a ffinity purified human iNOS antiserum and human IFN-gamma monoclonal antibo dies. Results The results revealed that iNOS-gamma producing cells localized adja cent to IFN-gamma-producing cells, In addition, some of iNOS-producing cell s exhibited immunoreactive IFN-gamma. On the other hand, epithelial cells s howed significant levels of iNOS production. but not IFN-gamma. Conclusions The data would suggest the possibility that INOS production cou ld be precisely controlled by autocrine or paracrine effects of IFN-gamma p roducing cells in radicular cysts and might play a pivotal role in periapic al lesions. These findings are consistent with a hypothesis suggesting that NO inhibitors could be used through the root canals as a pharmacological t reatment for periapical lesions.