In view of our preliminary findings that refined palm oil (RPOL) enhanced p
rotein utilisation, and because of the growing importance of palm oil in hu
man diets, two separate studies were conducted. The first study aimed to in
vestigate the effect of the palm oil source and fraction on protein utilisa
tion, as determined by net protein utilisation (NPU) in growing rats. The s
econd study aimed to investigate the influence of the dietary concentration
of one particular palm oil fraction, refined palm olein (RPO) on net prote
in utilisation. The fat source of each of the semi-purified diets was as fo
llows: crude palm oil (CPO), refined palm kernel oil (RKO), refined palm ol
ein (RPO), refined palm stearin (RPS), and refined palm oil (RPOL). A sixth
control group was offered an identical diet but with olive oil (OO, contro
l) as the fat source. Both the oil source (P < 0.01) and the oil level (P <
0.05) influenced NPU in rats. NPU values were generally higher for rats gi
ven the RPO-containing diets, particularly at the 20% dietary level (P < 0.
01) compared to the diets containing ground nut oil (GNO). The results of t
hese two experiments indicate that RPO influences protein metabolism specif
ically at a concentration of at least 20% in the diet. This may suggest tha
t RPO is superior to GNO as a source of fat for the rehabilitation of malno
urished children. Human trials notably during the rehabilitation of malnour
ished children may therefore be the next step in this line of research.