Vtw. Wilson et E. Cundliffe, Molecular analysis of tlrB, an antibiotic-resistance gene from tylosin-producing Streptomyces fradiae, and discovery of a novel resistance mechanism, J ANTIBIOT, 52(3), 1999, pp. 288-296
The tlrB gene. which confers inducible resistance to a range of macrolide a
ntibiotics including biosynthetic precursors of tylosin, was isolated and s
equenced. In the genome of Streptomyces fradiae it lies between pbp, which
encodes a putative penicillin-binding protein, and tylN, encoding a glycosy
ltransferase involved in tylosin biosynthesis. The TlrB protein was produce
d in E. coli as a fusion to MalE. The fusion protein, but not MalE alone, i
nactivates macrolides in the presence of S-adenosyl-methionine (SAM) but th
e modified product(s) has not been characterised.