Assay of tinidazole in human serum by high-performance thin-layer chromatography - Comparison with high-performance liquid chromatography

Determination of tinidazole in human serum by high-performance thin-layer c hromatography (HPTLC) is presented. It includes use of 10 x 10 cm plates co ated with silica gel 60 and chloroform-acetonitrile-acetic acid (60 + 40 2) as mobile phase. Quantitation was performed by densitometry at 320 nm, T he linearity (1-10 ng), precision (6%), reproducibility (5%), recovery (96% ), and detection limit (I mg/L) of tinidazole determination by HPTLC were c omparable with corresponding method parameters by reversed-phase HPLC. A sa tisfactory correlation was found between the 2 analytical methods. The proc edure was used to quantitate tinidazole in patient sera.