Determination of glycarbylamide is chicken tissue by high-performance liquid chromatography

A high-performance liquid chromatographic (HPLC) method for determining gly carbylamide (GB) in chicken tissue was developed. GB was extracted with ace tonitrile, followed by solid-phase extraction cleanup using a Bond Elut car tridge column with neutral alumina. After the. extract had been evaporated to dryness, the residue was dissolved in 1.0 mt 0.1N sodium hydroxide. Then 1.0 mt 0.1M potassium dihydrogen phosphate solution was added to it, HPLC separation was done on a 250 x 4.6 mm id TSK-GEL ODS 80(TM) column with 0.0 5M potassium dihydrogen phosphate as the mobile phase. Ultraviolet detectio n was done at a wavelength of 260 nm. The calibration curve of: standard GB solutions was linear between 0.16 and 3 mu g/mL (correlation coefficient, r = 0.999), The recovery of GB from chicken muscle spiked at 0.8 mu g/g was 88.6 +/- 2.3% (mean +/- standard deviation, n = 5), and the lower limit of determination was 0.05 mu g/g in chicken muscle.