Determination of biogenic amines by capillary electrophoresis

A method far determining biogenic amines in food using micellar electrokine tic capillary chromatography has been developed. Derivatization of the amin es was performed with AccQ (6-aminoquinolyl-N-hydroxysuccinimidyl carbamate ; Waters, Milford, MA, USA) reagent. The influence of buffer composition on the separation (including pH, SDS concentration and various additives) was investigated. The separation of seven biogenic amines (histamine, tyramine , tryptamine, spermine, spermidine, cadaverine acid putrescine) could be ac hieved within 25-30 min with good repeatability. The biogenic amine profile s in three different food samples (wine, salami and chive) were determined and quantitated. (C) 1999 Published by Elsevier Science B.V. All rights res erved.