Experiments were conducted to determine the influence of organic loading ra
te (OLR) and Yucca schidigera extract bioaugmentation on the performance of
attached-film anaerobic digesters under low temperature conditions (10 deg
rees C). The digesters were packed with layered media (polyester above lime
stone). Digester performance was evaluated with respect to biogas productio
n, methane production, and methane yield based on volatile solids added. Me
thane yield increased as OLR was reduced, apparently because of continued d
igestion of solids that had accumulated at the higher OLRs. After the OLR w
as reduced to its lowest value (0.01 kg VS/m(3).d), yield began decreasing
as stable operation was approached. Bioaugmentation appeared to improve met
hane production in a digester operated under stress (10 degrees C and a ver
y low OLR of 0.01 kg VS/m(3).d).